miRNA Tagging and Affinity-purification (miRAP)
نویسندگان
چکیده
منابع مشابه
Tandem affinity purification of miRNA target mRNAs (TAP-Tar)
MicroRNAs (miRNAs) bind to Argonaute proteins, and together they form the RISC complex and regulate target mRNA translation and/or stability. Identification of mRNA targets is key to deciphering the physiological functions and mode of action of miRNAs. In mammals, miRNAs are generally poorly homologous to their target sequence, and target identification cannot be based solely on bioinformatics....
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ژورنال
عنوان ژورنال: BIO-PROTOCOL
سال: 2012
ISSN: 2331-8325
DOI: 10.21769/bioprotoc.265